Following is a CAS database record representing a highly cited patent family.
|
CAS indexed 4 chemical substances from this document.
CAS subject entries for this document include: Deoxyribonucleic acid formation; Ribonucleic acid formation; Infection; and 9 additional concepts. | |
CAPLUS COPYRIGHT 2003 ACS
| TITLE:
| Amplifying, detecting, and/or cloning nucleic acid sequences by primer extension |
| INVENTOR(S):
| Mullis, Kary Banks; Arnheim, Norman; Saiki, Randall Keichi; Erlich, Henry Anthony; Horn, Glenn Thomas; Scharf, Stephen Joel |
| PATENT ASSIGNEE(S):
| Cetus Corp., USA |
| SOURCE:
| Eur. Pat. Appl., 45 pp. CODEN: EPXXDW |
| LANGUAGE:
| English |
PATENT INFORMATION: PATENT NO. KIND DATE APPLICATION NO. DATE
--------------- ---- -------- --------------- --------
EP 200362 A2 19861210 EP 1986-302298 19860327
EP 200362 A3 19870225
EP 200362 B1 19930120
R: AT, BE, CH, DE, FR, GB, IT, LI, LU, NL, SE
US 4683202 A 19870728 US 1985-791308 19851025
US 4683195 A 19870728 US 1986-828144 19860207
DK 8601448 A 19860929 DK 1986-1448 19860326
DK 171160 B1 19960708
DK 8601449 A 19860929 DK 1986-1449 19860326
DK 171161 B1 19960708
ES 553464 A1 19870616 ES 1986-553464 19860326
ES 553468 A1 19870616 ES 1986-553468 19860326
AU 8655322 A1 19861002 AU 1986-55322 19860327
AU 586233 B2 19890706
AU 8655323 A1 19861002 AU 1986-55323 19860327
AU 591104 B2 19891130
EP 201184 A2 19861217 EP 1986-302299 19860327
EP 201184 A3 19870225
EP 201184 B1 19921216
R: AT, BE, CH, DE, FR, GB, IT, LI, LU, NL, SE
ZA 8602334 A 19871125 ZA 1986-2334 19860327
ZA 8602335 A 19871125 ZA 1986-2335 19860327
CA 1237685 A1 19880607 CA 1986-505375 19860327
IL 78281 A1 19910610 IL 1986-78281 19860327
IL 78284 A1 19910916 IL 1986-78284 19860327
CA 1291429 A1 19911029 CA 1986-505395 19860327
EP 502588 A2 19920909 EP 1992-201226 19860327
EP 502588 A3 19940119
R: AT, BE, CH, DE, FR, GB, IT, LI, LU, NL, SE
EP 502589 A2 19920909 EP 1992-201245 19860327
EP 502589 A3 19940119
EP 502589 B1 20010926
R: AT, BE, CH, DE, FR, GB, IT, LI, LU, NL, SE
EP 505012 A2 19920923 EP 1992-201244 19860327
EP 505012 A3 19940119
EP 505012 B1 19980506
R: AT, BE, CH, DE, FR, GB, IT, LI, LU, NL, SE
EP 509612 A2 19921021 EP 1992-201243 19860327
EP 509612 A3 19940119
EP 509612 B1 20010926
R: AT, BE, CH, DE, FR, GB, IT, LI, LU, NL, SE
AT 83505 E 19930115 AT 1986-302299 19860327
AT 84822 E 19930215 AT 1986-302298 19860327
AT 165869 E 19980515 AT 1992-201244 19860327
AT 206169 E 20011015 AT 1992-201243 19860327
AT 206168 E 20011015 AT 1992-201245 19860327
JP 61274697 A2 19861204 JP 1986-68858 19860328
JP 04067960 B4 19921029
JP 62000281 A2 19870106 JP 1986-68857 19860328
JP 04067957 B4 19921029
ES 557287 A1 19871016 ES 1987-557287 19870109
ES 557288 A1 19871016 ES 1987-557288 19870109
US 5176995 A 19930105 US 1989-394145 19890815
US 4683195 B1 19901127 US 1989-90001902 19891206
US 4683202 B1 19901127 US 1989-90001903 19891206
JP 05308971 A2 19931122 JP 1992-177779 19920612
JP 06007166 A2 19940118 JP 1992-177761 19920612
JP 2546576 B2 19961023
US 5386022 A 19950131 US 1993-92767 19930716
US 5656493 A 19970812 US 1994-199505 19940218
US 5594123 A 19970114 US 1994-287385 19941024
DK 9500265 A 19950317 DK 1995-265 19950317
DK 171871 B1 19970721
CA 1340121 B 19981110 CA 1995-617031 19951026
DK 9600132 A 19960209 DK 1996-132 19960209
DK 9600133 A 19960209 DK 1996-133 19960209
ABSTRACT:
A method for amplifying nucleic acid sequences present or suspected to be present in a sample comprises (1) treating sep. complementary strands of the nucleic acid with two oligonucleotide primers; and (2) extending the primers to form complementary extended products which act as templates for the synthesis of the desired nucleic acid sequence. Amplification of a fragment of the human Hb .beta.-chain gene was performed by treating a human genomic DNA sample from the blood of a sickle cell carrier with an excess of oligonucleotide primers d(CACAGGGCAGTAACG) and d(TTTGCTTCTGACACA) for hybridization and extending the primers in the presence of dATP, dCTP, dGTP, TTP, and the Klenow fragment. A hybridization assay showed that the amplification increased sickle-cell anemia detection sensitivity by .gtoreq.1000 fold.
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