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Home   •   Spotlight  •  pcite00  •  Most Cited Patent Families 2000-Chemistry and Related (2)

Most Cited Patent Families 2000-Chemistry and Related

Following is a CAS database record representing a highly cited patent family.

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CAS indexed 39 chemical substances from this document. CAS subject entries for this document include: Glass, oxide; Protective groups; Immobilization, biochemical; and 9 additional concepts.

CAPLUS COPYRIGHT 2001 ACS

TITLE:	Large-scale photolithographic solid-phase synthesis of polypeptides and receptor binding screening thereof
INVENTOR(S):	Pirrung, Michael C.; Read, J. Leighton; Fodor, Stephen P. A.; Stryer, Lubert
PATENT ASSIGNEE(S):	Affymax Technologies N. V., Neth. Antilles
SOURCE:	U.S., 40 pp. Cont.-in-part of U.S. Ser. No. 362,901, abandoned. CODEN: USXXAM
LANGUAGE:	English

PATENT INFORMATION:

PATENT NO.       KIND  DATE           APPLICATION NO.  DATE
---------------  ----  --------       ---------------  --------
US 5143854        A    19920901       US 1990-492462   19900307
IL 94551          A1   19950330       IL 1990-94551    19900529
ZA 9004354        A    19910828       ZA 1990-4354     19900606
CA 2054706        AA   19901208       CA 1990-2054706  19900607
WO 9015070        A1   19901213       WO 1990-NL81     19900607
    W:  AT, AU, BB, BG, BR, CA, CH, DE, DK, ES, FI, GB, HU, JP, KP, KR,
	LK, LU, MC, MG, MW, NL, NO, RO, SD, SE, SU
    RW: AT, BE, BF, BJ, CF, CG, CH, CM, DE, DK, ES, FR, GA, GB, IT, LU,
	ML, MR, NL, SE, SN, TD, TG
AU 9058371        A1   19910107       AU 1990-58371    19900607
AU 651795         B2   19940804
NL 9022056        A    19920302       NL 1990-22056    19900607
NL 191992         B    19960801NL 191992         C    19961203
EP 476014         A1   19920325       EP 1990-909187   19900607
EP 476014         B1   19940831
    R:  AT, BE, CH, DE, DK, ES, FR, GB, IT, LI, LU, NL, SE
BR 9007425        A    19920721       BR 1990-7425     19900607
HU 59938          A2   19920728       HU 1990-4730     19900607
JP 04505763       T2   19921008       JP 1990-508966   19900607
EP 619321         A1   19941012       EP 1994-200059   19900607
EP 619321         B1   19990107
    R:  AT, BE, CH, DE, DK, ES, FR, GB, IT, LI, LU, NL, SE
ES 2058921        T3   19941101       ES 1990-909187   19900607
AT 175421         E    19990115       AT 1994-200059   19900607
JP 11021293       A2   19990126       JP 1996-324451   19900607
EP 902034         A2   19990317       EP 1998-203518   19900607
EP 902034         A3   19990526
    R:  AT, BE, CH, DE, DK, ES, FR, GB, IT, LI, LU, NL, SE
ES 2129101        T3   19990601       ES 1994-200059   19900607
EP 953835         A1   19991103       EP 1999-202455   19900607
    R:  AT, BE, CH, DE, DK, ES, FR, GB, IT, LI, LU, NL, SE
JP 11315095       A2   19991116       JP 1999-49217    19900607
US 5547839        A    19960820       US 1990-626730   19901206
NO 9104826        A    19911206       NO 1991-4826     19911206
GB 2248840        A1   19920422       GB 1991-25996    19911206
GB 2248840        B2   19931201
US 5424186        A    19950613       US 1991-805727   19911206
KR 9701577        B1   19970211       KR 1991-71791    19911206
US 5405783        A    19950411       US 1992-850356   19920312
US 5445934        A    19950829       US 1992-954646   19920930
US 5510270        A    19960423       US 1992-954519   19920930
US 5527681        A    19960618       US 1992-972007   19921105
AU 9477655        A1   19950504       AU 1994-77655    19941104
AU 672723         B2   19961010
US 5744101        A    19980428       US 1995-388321   19950214
US 5489678        A    19960206       US 1995-390272   19950216
US 5753788        A    19980519       US 1995-446177   19950519
US 5889165        A    19990330       US 1995-444598   19950519
US 6225625        B1   20010501       US 1995-456598   19950601
US 5744305        A    19980428       US 1995-466632   19950606
US 5770456        A    19980623       US 1996-647618   19960513
US 5800992        A    19980901       US 1996-670118   19960625
US 5902723        A    19990511       US 1996-679478   19960712
KR 9701578        B1   19970211       KR 1996-75897    19961021
US 5871928        A    19990216       US 1997-873034   19970611
US 5925525        A    19990720       US 1998-54420    19980403
US 6197506        B1   20010306       US 1998-56927    19980408
US 6124102        A    20000926       US 1998-63936    19980421

ABSTRACT:
Polypeptide arrays can be synthesized on a substrate by attaching photoremovable groups to the surface of a substrate, exposing selected regions of the substrate to light to activate those regions, attaching an amino acid monomer with a photoremovable group to the activated regions, and repeating the steps of activation and attachment until polypeptides of the desired length and sequences are synthesized. The resulting array can be used to det. which peptides on the array can bind to a receptor, e.g. an antibody. A slide was derivatized with aminopropyltriethoxysilane and protected with a nitroveratryloxycarbonyl protecting group. A 500 .mu.m checkerboard mask was used to expose the slide in a flow cell using backside contact printing. Leu enkephalin (YGGFL) was attached via its carboxyl end to the exposed amino groups on the surface of the slide. Binding of the peptide by an antibody (Herz antibody) was detected using a 2nd, goat anti-mouse fluoresceinated antibody. Similar techniques were used to test binding of the Herz antibody to arrays of differing sequences.

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