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Most Cited Patent Families 2000-Chemistry and Related

Following is a CAS database record representing a highly cited patent family.

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CAS indexed 4 chemical substances from this document. CAS subject entries for this document include: Deoxyribonucleic acid formation; Ribonucleic acid formation; Infection; and 9 additional concepts.

CAPLUS COPYRIGHT 2001 ACS

TITLE:	Amplifying, detecting, and/or cloning nucleic acid sequences by primer extension
INVENTOR(S):	Mullis, Kary Banks; Arnheim, Norman; Saiki, Randall Keichi; Erlich, Henry Anthony; Horn, Glenn Thomas; Scharf, Stephen Joel
PATENT ASSIGNEE(S):	Cetus Corp., USA
SOURCE:	Eur. Pat. Appl., 45 pp. CODEN: EPXXDW
LANGUAGE:	English

PATENT INFORMATION:

PATENT NO.       KIND  DATE           APPLICATION NO.  DATE
---------------  ----  --------       ---------------  --------
EP 200362         A2   19861210       EP 1986-302298   19860327
EP 200362         A3   19870225EP 200362         B1   19930120
    R:  AT, BE, CH, DE, FR, GB, IT, LI, LU, NL, SE
US 4683202        A    19870728       US 1985-791308   19851025
US 4683195        A    19870728       US 1986-828144   19860207
DK 8601448        A    19860929       DK 1986-1448     19860326
DK 171160         B1   19960708
DK 8601449        A    19860929       DK 1986-1449     19860326
DK 171161         B1   19960708
ES 553464         A1   19870616       ES 1986-553464   19860326
ES 553468         A1   19870616       ES 1986-553468   19860326
AU 8655322        A1   19861002       AU 1986-55322    19860327
AU 586233         B2   19890706
AU 8655323        A1   19861002       AU 1986-55323    19860327
AU 591104         B2   19891130
EP 201184         A2   19861217       EP 1986-302299   19860327
EP 201184         A3   19870225EP 201184         B1   19921216
    R:  AT, BE, CH, DE, FR, GB, IT, LI, LU, NL, SE
ZA 8602334        A    19871125       ZA 1986-2334     19860327
ZA 8602335        A    19871125       ZA 1986-2335     19860327
CA 1237685        A1   19880607       CA 1986-505375   19860327
IL 78281          A1   19910610       IL 1986-78281    19860327
IL 78284          A1   19910916       IL 1986-78284    19860327
CA 1291429        A1   19911029       CA 1986-505395   19860327
EP 502588         A2   19920909       EP 1992-201226   19860327
EP 502588         A3   19940119
    R:  AT, BE, CH, DE, FR, GB, IT, LI, LU, NL, SE
EP 502589         A2   19920909       EP 1992-201245   19860327
EP 502589         A3   19940119
    R:  AT, BE, CH, DE, FR, GB, IT, LI, LU, NL, SE
EP 505012         A2   19920923       EP 1992-201244   19860327
EP 505012         A3   19940119EP 505012         B1   19980506
    R:  AT, BE, CH, DE, FR, GB, IT, LI, LU, NL, SE
EP 509612         A2   19921021       EP 1992-201243   19860327
EP 509612         A3   19940119
    R:  AT, BE, CH, DE, FR, GB, IT, LI, LU, NL, SE
AT 83505          E    19930115       AT 1986-302299   19860327
AT 84822          E    19930215       AT 1986-302298   19860327
AT 165869         E    19980515       AT 1992-201244   19860327
JP 61274697       A2   19861204       JP 1986-68858    19860328
JP 04067960       B4   19921029
JP 62000281       A2   19870106       JP 1986-68857    19860328
JP 04067957       B4   19921029
ES 557287         A1   19871016       ES 1987-557287   19870109
ES 557288         A1   19871016       ES 1987-557288   19870109
US 5176995        A    19930105       US 1989-394145   19890815
US 4683195        B1   19901127       US 1989-90001902 19891206
US 4683202        B1   19901127       US 1989-90001903 19891206
JP 05308971       A2   19931122       JP 1992-177779   19920612
JP 06007166       A2   19940118       JP 1992-177761   19920612
JP 2546576        B2   19961023
US 5386022        A    19950131       US 1993-92767    19930716
US 5656493        A    19970812       US 1994-199505   19940218
US 5594123        A    19970114       US 1994-287385   19941024
DK 9500265        A    19950317       DK 1995-265      19950317
DK 171871         B1   19970721
CA 1340121        B    19981110       CA 1995-617031   19951026
DK 9600132        A    19960209       DK 1996-132      19960209
DK 9600133        A    19960209       DK 1996-133      19960209

ABSTRACT:
A method for amplifying nucleic acid sequences present or suspected to be present in a sample comprises (1) treating sep. complementary strands of the nucleic acid with two oligonucleotide primers; and (2) extending the primers to form complementary extended products which act as templates for the synthesis of the desired nucleic acid sequence. Amplification of a fragment of the human Hb .beta.-chain gene was performed by treating a human genomic DNA sample from the blood of a sickle cell carrier with an excess of oligonucleotide primers d(CACAGGGCAGTAACG) and d(TTTGCTTCTGACACA) for hybridization and extending the primers in the presence of dATP, dCTP, dGTP, TTP, and the Klenow fragment. A hybridization assay showed that the amplification increased sickle-cell anemia detection sensitivity by .gtoreq.1000 fold.

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