• CAS Science Spotlight Home
• Most Cited Journal Articles 1999-Medical Sciences
• About
• What's New

Home   •   Spotlight  •  medsci99  •  Most Cited Journal Articles 1999-Medical Sciences (8)

Most Cited Journal Articles 1999-Medical Sciences

 

Following is a CAS database record representing a highly cited journal article.

---

CAS indexed 3 chemical substances from this document. CAS subject entries for this document include: Artery, metabolism.

CAPLUS COPYRIGHT 2002 ACS

TITLE:	Nitric oxide release accounts for the biological activity of endothelium-derived relaxing factor
AUTHOR(S):	Palmer, R. M. J.; Ferrige, A. G.; Moncada, S.
CORPORATE SOURCE:	Wellcome Res. Lab., Beckenham/Kent, BR3 3BS, UK
SOURCE:	Nature (London) (1987), 327(6122), 524-6 CODEN: NATUAS; ISSN: 0028-0836
PUBLISHER:	Macmillan Magazines
LANGUAGE:	English

ABSTRACT:
The release of endothelium-derived relaxing factor (EDRF) and NO from porcine aortic endothelial cells in culture was examd. NO was detd. as the chemiluminescent product of its reaction with ozone. The biol. activity of EDRF and of NO was measured by bioassay. The relaxation of the bioassay tissues induced by EDRF was indistinguishable from that induced by NO. Both substances were equally unstable. Bradykinin caused concn.-dependent release of NO from the cells in amts. sufficient to account for the biol. activity of EDRF. The relaxations induced by EDRF and NO were inhibited by Hb and enhanced by superoxide dismutase to a similar degree. Thus, NO released from endothelial cells is indistinguishable from EDRF in terms of biol. activity, stability, and susceptibility to an inhibitor and to a potentiator. EDRF and NO are probably identical.

View the full-text pdf document from the Nature Publishing Group.