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Most Cited Journal Articles 2000-Medical Sciences
Following is a CAS database record representing a highly cited journal article.
CAPLUS COPYRIGHT 2002 ACS
Mutation of the obese (ob) gene in mice results in profound obesity and type II diabetes as part of a syndrome that resembles morbid obesity in humans. The ob gene was subjected to positional cloning, and the sequences of 22 ob cDNAs from a mouse adipose tissue cDNA library was detd. A 97-bp 5'-leader was followed by a predicted 167-amino acid ORF and a ~3700-kb 3'-untranslated sequence. The predicted protein had a putative N-terminal signal sequence. Microheterogeneity was found with cDNAs differing by inclusion or exclusion of a single glutamine codon, apparently due to slippage at the splice-acceptor site. C57BL/6J ob/ob mice had a C.fwdarw.T mutation that resulted in a change of Arg-105 codon to a stop codon. The SM/Ckc-+Dacob2J/ob2J mice seemed to have a mutation in the promoter region of this gene. In vitro expts. suggested that the encoded protein can undergone signal sequence cleavage and secretion. With the mouse gene as a probe, human adipose tissue cDNA clones of the human homolog of the ob gene were obtained and sequenced. The human protein was highly homologous to the mouse protein. View the full-text pdf document from the Nature Publishing Group. Updated 4/18/2007 2:43:34 PM
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