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Most Cited Journal Articles 1999-Chemistry and Related

Following is a CAS database record representing a highly cited journal article.

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CAS indexed 3 chemical substances from this document. CAS subject entries for this document include: Animal tissue; Cell; Ribonucleic acids; and 2 additional concepts.

CAPLUS COPYRIGHT 2001 ACS

TITLE:	Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction
AUTHOR(S):	Chomczynski, Piotr; Sacchi, Nicoletta
CORPORATE SOURCE:	Lab. Biochem. Metabol., NIDDK, Bethesda, MD, 20892, USA
SOURCE:	Analytical Biochemistry (1987), 162(1), 156-9 CODEN: ANBCA2; ISSN: 0003-2697
PUBLISHER:	Academic Press
LANGUAGE:	English

ABSTRACT:
A new method of total RNA isolation by a single extn. with an acid guanidinium thiocyanate-phenol-chloroform mixt. is described. The method provides a pure prepn. of undegraded RNA in high yield and can be completed within 4 h. It is particularly useful for processing large nos. of samples and for isolation of RNA from minute quantities of cells or tissue samples. Academic Press