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Most Cited Journal Articles 2004-Chemistry and Related

Following is a CAS database record representing a highly cited journal article.

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CAS subject entries for this document include: Deoxyribonucleic acid sequences; Protein sequences; Algorithm .

CAPLUS COPYRIGHT 2005 ACS on STN

TITLE:	Basic local alignment search tool
AUTHOR(S):	Altschul, Stephen F.; Gish, Warren; Miller, Webb; Myers, Eugene W.; Lipman, David J.
CORPORATE SOURCE:	Natl. Cent. Biotechnol. Inf., Natl. Library Med., Bethesda, MD, 20894, USA
SOURCE:	Journal of Molecular Biology (1990), 215(3), 403-10 CODEN: JMOBAK; ISSN: 0022-2836
PUBLISHER:
LANGUAGE:	English

ABSTRACT:
A new approach to rapid sequence comparison, basic local alignment search tool (BLAST), directly approximates alignments that optimize a measure of local similarity, the maximal segment pair (MSP) score. Recent math. results on the stochastic properties of MSP scores allow an anal. of the performance of this method as well as the statistical significance of alignments it generates. The basic algorithm is simple and robust; it can be implemented in a no. of ways and applied in a variety of contexts including straightforward DNA and protein sequence database searches, motif searches, gene identification searches, and in the anal. of multiple regions of similarity in long DNA sequences. In addn. to its flexibility and tractability to math. anal., BLAST is an order of magnitude faster than existing sequence comparison tools of comparable sensitivity.