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Most Cited Journal Articles 2002-Chemistry and Related

Following is a CAS database record representing a highly cited journal article.

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CAS subject entries for this document include: Proteins .

CAPLUS COPYRIGHT 2003 ACS

TITLE:	Protein measurement with the Folin phenol reagent
AUTHOR(S):	Lowry, Oliver H.; Rosebrough, Nira J.; Farr, A. Lewis; Randall, Rose J.
CORPORATE SOURCE:	Washington Univ., St. Louis, MO
SOURCE:	Journal of Biological Chemistry (1951), 193, 265-75 CODEN: JBCHA3; ISSN: 0021-9258
PUBLISHER:	The American Society for Biochemistry and Molecular Biology
LANGUAGE:	English

ABSTRACT:
cf. C.A. 41, 1271h. Proteins were detd. with the Folin phenol reagent after alk. Cu treatment. The method is as sensitive as with Nessler reagent, yet requires no digestion. It is 10-20 times more sensitive than detn. of the ultraviolet absorption at .lambda. = 280 m.mu. and is more specific. It is several fold more sensitive than the ninhydrin reaction and 100 times more sensitive than the biuret reaction. Two major disadvantages are: the amt. of color varies with different proteins; the color is not strictly proportional to concn. Few substances cause serious interference. Uric acid, guanine, and xanthine react with Folin reagent. The method is useful for following enzyme fractionation, detg. mixed tissue proteins, detn. of very small amts. of protein, or detg. highly dild. protein, or protein in colored solns. or in the presence of N-contg. material.

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