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Most Cited Journal Articles 2002-Chemistry and Related

Following is a CAS database record representing a highly cited journal article.

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CAS subject entries for this document include: Proteins .

CAPLUS COPYRIGHT 2003 ACS

TITLE:	A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
AUTHOR(S):	Bradford, Marion M.
CORPORATE SOURCE:	Dep. Biochem., Univ. Georgia, Athens, GA, USA
SOURCE:	Analytical Biochemistry (1976), 72(1-2), 248-54 CODEN: ANBCA2; ISSN: 0003-2697
PUBLISHER:	Academic Press
LANGUAGE:	English

ABSTRACT:
A protein detn. method that involves the binding of coomassie Brilliant Blue G 250 to protein is described. The binding of the dye to protein causes a shift in the absorption max. of the dye from 465 to 595 nm, and it is the increase in absorption at 595 nm that is monitored. This assay is very reproducible and rapid with the dye binding process virtually complete in .apprx. 2 min with good color stability for 1 hr. There is little or no interference from cations such as Na+ or K+ nor from carbohydrates such as sucrose. A small amt. of color is developed in the presence of strongly alk. buffering agents, but the assay may be run accurately by the use of proper buffer controls. The only components found to give excessive interfering color in the assay are relatively large amts. of detergents such as Na dodecyl sulfate, Triton X 100, and commercial glassware detergents. Interference by small amts. of detergent may be eliminated by the use of proper control.