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Most Cited Journal Articles 2000-Chemistry and Related
Following is a CAS database record representing a highly cited journal article.
CAPLUS COPYRIGHT 2001 ACS
A method was devised for the electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets. The method results in quant. transfer of ribosomal proteins from gels contg. urea. For Na dodecyl sulfate gels, the original band pattern was obtained with no loss of resoln., but the transfer was not quant. The method allows detection of proteins by autoradiog. and is simpler than conventional procedures. The immobilized proteins were detectable by immunol. procedures. All addnl. binding capacity on the nitrocellulose was blocked with excess protein; then, a specific antibody was bound and, finally, a 2nd antibody directed against the 1st antibody. The 2nd antibody was wither radioactively labeled or conjugated to fluorescein or to peroxidase. The specific protein was then detected by either autoradiog., under UV light, or by the peroxidase reaction product, resp. In the latter case, as little as 100 pg of protein was clearly detectable. Updated 4/18/2007 9:33:59 AM
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