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Most Cited Journal Articles 2000-Chemistry and Related

 

Following is a CAS database record representing a highly cited journal article.

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CAS indexed 8 chemical substances from this document. CAS subject entries for this document include: Deoxyribonucleic acids; Nucleotides, properties; Virus, bacterial.

CAPLUS COPYRIGHT 2001 ACS

TITLE:	DNA sequencing with chain-terminating inhibitors
AUTHOR(S):	Sanger, F.; Nicklen, S.; Coulson, A. R.
CORPORATE SOURCE:	Lab. Mol. Biol., MRC, Cambridge, Engl.
SOURCE:	Proceedings of the National Academy of Sciences of the United States of America (1977), 74(12), 5463-7 CODEN: PNASA6; ISSN: 0027-8424
PUBLISHER:	National Academy of Sciences
LANGUAGE:	English

ABSTRACT:
A new method for detg. nucleotide sequences in DNA is described. It is similar to the plus and minus method (Sanger, F., Coulson, A. R., 1975) but makes use of the 2',3'-dideoxy and arabinonucleoside analogs of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase. The technique was applied to the DNA of bacteriophage .phi.X174 and was more rapid and more accurate than either the plus or the minus method.

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