Following is a CAS database record representing a highly cited journal article.
|
CAS indexed 3 chemical substances from this document.
CAS subject entries for this document include: Animal tissue; Cell; Ribonucleic acids; and 2 additional concepts. | |
CAPLUS COPYRIGHT 2001 ACS
| TITLE:
| Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction |
| AUTHOR(S):
| Chomczynski, Piotr; Sacchi, Nicoletta |
| CORPORATE SOURCE:
| Lab. Biochem. Metabol., NIDDK, Bethesda, MD, 20892, USA |
| SOURCE:
| Analytical Biochemistry (1987), 162(1), 156-9 CODEN: ANBCA2; ISSN: 0003-2697 |
| PUBLISHER:
| Academic Press |
| LANGUAGE:
| English |
ABSTRACT:
A new method of total RNA isolation by a single extn. with an acid guanidinium thiocyanate-phenol-chloroform mixt. is described. The method provides a pure prepn. of undegraded RNA in high yield and can be completed within 4 h. It is particularly useful for processing large nos. of samples and for isolation of RNA from minute quantities of cells or tissue samples. © Academic Press
Updated 4/18/2007 9:28:39 AM
