Requirement: Microsoft Windows® XP or higher.
Find subject sequences that are similar to your nucleotide or protein sequence by entering a:
Before You Begin
To search for similar nucleotide or protein sequences:
Access the Result Set Manager in CAS Registry BLAST.
Access your search type:
To search by: |
Use either of these methods: |
||
---|---|---|---|
From the Toolbar |
From the menu bar |
||
Sequence |
Click the Sequence
icon.
The Search by Sequence window displays. |
Select Search >
Similar Sequences.
The Search by Sequence window displays. |
|
Click the Sequence ID
icon.
The Similar Sequence using Sequence Identifier window displays. |
Select Search >
Similar Sequence using Sequence
Identifier.
Similar Sequence using Sequence Identifier window displays. |
(Optional) Enter a Result Name. Otherwise, a default name will be assigned.
Default name format: Result - nnnnnn
Enter your query.
Size restriction: All queries are restricted to 50,000 characters.
For this Explore type: |
Follow these guidelines: |
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Sequence |
Enter your sequence in the appropriate format (FASTA, bare sequence, GCG) and follow these guidelines:
|
Type or paste a single number in a supported format:
|
Select the BLAST program for your query.
The available protein or nucleotide database subset choices display, as appropriate.
Accept the default (all available subsets) or choose which database subsets to search.
If desired, choose Only retrieve sequences with 50 residues or less.
When to use: When you have a short query sequence and want to ensure that you see the shorter answers (subject sequences) that are returned.
Short queries can match both long and short sequences, producing many answers. This ensures that matches are limited to short sequences.
E value: If you select this option, adjust your E value as discussed in Searching Short Sequences.
Restrictions: This option is NOT available:
Click OK.
The Settings window for your program displays.
Accept the Basic Options defaults.
– or –
Choose new values (as appropriate) for the basic
options that apply to your program.
Select Show Additional Options to adjust the additional options as appropriate, or accept the default values.
Click OK to submit your search.
When a search is submitted, CAS Registry BLAST:
Creates a sequence query (and queues it if necessary).
Performs the search, indicating its status in the Result Set Manager. When the status column indicates Complete, your result is available.
Closing the window: Closing the Result Set Manager window during a running search does not affect its status. The search continues to run in the background.
Automatically saves your result set in the Result Set Manager.
Maximum: You can keep a maximum of 100 search queries in the Result Set Manager, regardless of the search status.
From the Result Set Manager, you can perform these and other functions:
You can also manipulate your results or the appearance of the listing in the Result Set Manager:
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See also
Reports Tab (Result Set Manager)
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BLAST® is a registered trademark of the National Library of Medicine.
BLAST® reference information provided in whole or in part from the National Center for Biotechnology Information (NCBI), National Library of Medicine, National Institutes of Health.
Unless designated otherwise, all other information Copyright © 1997-2017 by the American Chemical Society. All rights reserved.